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An assay that lacks sensitivity at low cell numbers or introduces variability between plates, will undermine every downstream result

Aging (Albany NY) 2019;11, 771782, doi: Zhang, X

10.3390/gels7030096 68 LeeM.YangC.SongG.LimW

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And by the same token, if theres issues in methylation anywhere, not just MTHFR, but and the whole folate methionine cycle, were gonna see a compromised level of methyl donors being produced, which means histamine and methyltransferase doesnt have the conjugates to be able to clear histamine in the first place, so it is a bit of a wicked web to go down