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Loren Pickart in 1973, the GHK-Cu copper peptide was isolated based on observations that plasma from younger individuals contained factors that influenced liver cell behavior differently than plasma from older individuals

Winger and Zamvil, 2016)

Tubulin expression was detected on the same PVDF membrane using anti--tubulin specific monoclonal antibody (1 g/ml Sigma-Aldrich, Ont., CA), followed by HRP-conjugated goat anti-mouse IgG (1:5000 (v/v), BioRad, Ont., CA)

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