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48 hours after siRNA transfection, cells were detached and harvested with 0.25% Trypsin-EDTA (Gibco) and washed with PBS

In cultured astrocytes, TDP-43 elevates IL-1, IL-6, and TNF- levels, mirroring ALS patient blood profiles and stimulating microglial proliferation via NF-B activation [250, 251]

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