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GLP-1 is the only known ligand for GLP-1R, a Gs-protein coupled receptor (GPCR), which can activate a multitude of downstream pathways including cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA), cAMP/guanine-nucleotide exchange factor, and phosphatidylinositol-3 kinase/Protein Kinase C

3.1.5 Statistical analysis The primary efficacy outcome was determined by the standardized mean difference (SMD) in validated symptom scale scores between baseline and post-treatment assessments

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Statistical analysis All measurements in cell culture were carried out, at least, in triplicate, and the results are expressed as the means.e.m

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