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The secreted IL-10 cytokine values measured in the supernatant collected from cells stimulated with LPS and treated with the test products are reported in Table 3, which are expressed in terms of percentage (%) with respect to the cytokine level measured in the supernatant collected from control cells, stimulated with LPS, and incubated with the medium alone

Conditioned medium was extracted by taking 10 l of the medium supernatant and mixing it with 390 l of the extraction solvent

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1 2 America's Obesity Crisis: The Health and Economic Costs of Excess Weight