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After washing twice with PBS, the eluate (50 mM Tris-HCl (pH 7.8) and 10 mM glutathione) was dialyzed with PBS

China Patent CN102492591 (B) Xiong Z, Guo M, Guo Y, Chu J, Zhuang Y, Zhang S (2008) Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium