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One thousand ng of total RNA was reverse transcribed into complementary DNA (cDNA) using the SensiFAST cDNA Synthesis Kit (Bioline, UK), following the manufacturer`s instructions

The six-membered ring to which the -lactam is fused in cephalosporins, results in lower tension within the -lactam ring compared to those of penicillins, and therefore less reactivity against thiolate anions (Perez-Inestrosa et al., 2005)

doi: 10.1016/j.bbagen.2012.11.020 19 YangWSSriRamaratnamRWelschMEShimadaKSkoutaRViswanathanVSet al

Ann Intern Med (1985) 102:17