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The Natural Products Journal, 8 (1), 3244
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Protein purification Cell pellets were resuspended in ice-cold lysis buffer containing 20 mM Tris-Cl (pH 8.0), 150 mM NaCl, 0.2 mM PMSF, 20 g/mL DNase I, and 20 M DNQX
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Obestatin , a 23 amino acid amidated peptide, is derived from preproghrelin, which is the same precursor as ghrelin (Figure 3)
