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Studies indicate that activation of TLR2/3/4/5 triggers the NF-B/NLRP3 pathway and the PI3K/Akt/mTORC1 signaling pathway, leading to microglial activation, neuroinflammation, and neuronal damage (147, 148)

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While any peptides or proteins may theoretically trigger immune reactions, therapeutic formulations are carefully purified and optimized to minimize this risk

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A key distinction maintained throughout this guide is delivery route: GHK-Cu serum and topical formulations hold the strongest evidence base, while GHK-Cu injection protocols remain practitioner-reported and research-extrapolated