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A 2021 systematic review of long-term studies found robust effects of physical exercise on dopamine persisting across the lifespan

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The samples were then centrifuged at 18 000 g at 4 C for 15 min, and 20 l of the resulting supernatants was injected into the HPLC column for metabolite quantification, while the pellet was used for protein analysis using the BCA protein assay

REGULATION OF NITRIC OXIDE PRODUCTION IN HEALTH AND DISEASE. Current opinion in clinical nutrition and metabolic care 13.1 (2010): 97104