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Protein pellets were suspended and denatured in 20 l 7 M urea/2 M thiourea/100 mM TEAB, pH 7.5, reduced with 2 L of 50 mM Tris(2-carboxyethyl) phosphine (TCEP, SCIEX), pH 8.0, at 37 C for 60 min and followed by the addition of 1 L of 200 mM cysteine-blocking reagent (methyl methanethiosulfonate (MMTS, Pierce) for 10 min at room temperature
3b) are shown for the nucleic acid and enzyme samples
To view a copy of this license, visit About this article Cite this article Petukhova, V.Z., Aboagye, S.Y., Ardini, M
Increased ROS levels can activate NF-B and MAPK, and then activate psoriasis Th1 and Th17 cells (134)
Data availability The RNA array data generated in this study have been deposited in the Gene Expression Omnibus (GEO) database under accession code GSE203657