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Reusing a needle introduces contamination from the previous injection site and increases coring risk from a blunted needle tip

Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

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However, both branches of the same limb may be affected, which is more common in the hind limbs

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