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Chl content and ALA-synthesizing capacity consistently remained wild-type-like ( Supplementary Figures 3B, C ) with the striking exception of GSAAT(C190S), which exhibited a significantly lower rate of ALA synthesis

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The fluorescence reading was performed on the GloMax Explorer plate reader (Promega) using excitation and emission filters of 520 nm and 580 to 640 nm (green), respectively

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