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Diabetes Therapy, 12(2), 783-794

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New functions of aminoacyl-tRNA synthetases beyond translation

Protein extracts (1575 g) were boiled at 95 C for 5 min in loading buffer (100 mM Tris pH 6.8, 10% glycerol, 4% sodium dodecyl sulfate (SDS), 0.2% bromophenol blue and 2 mM -mercaptoethanol) and subjected to 812% SDS polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene difluoride (PVDF) membranes