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In contrast, if metabolically competent human HepaRG cells [142] or primary human hepatocytes [46] were treated with 20 mM APAP for 24 h, there was 8090% GSH depletion, a ten-fold higher protein adducts formation, 7090% reduction of the mitochondrial membrane potential and 610-fold increase in LDH or ALT release

Importantly, AOD 9604 has been studied for a favorable safety profile, showing minimal immunogenicity in experimental use