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The concentration of the working solution was 20 L per milliliter of PBS, which was left at room temperature for 25 min before observing under a fluorescence microscope

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doi: 10.1016/J.JDDST.2020.102126 94 KhanS.TndervikA.SlettaH.KlinkenbergG.EmanuelC.OnsyenE.et al

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7.Withdraw the needle from the vial