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Western blot analysis Proteins were extracted and estimated from the bones samples using RIPA lysis buffer (150 mM NaCl, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulfate), 50 mM TrisHCl, pH was adjusted at 8.0, 0.4 l protease inhibitor buffer, 2.5 l phosphatase inhibitor) from BufferBio BASIC Inc
Peer review Peer review information Nature Chemical Biology thanks Melanie McReynolds, Yi Yang and the other, anonymous, reviewer(s) for their contribution to the peer review of this work
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