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Western blotting The HepG2 cells (2 10 6 cells per dish) were seeded in 10 cm culture dishes and incubated for 24 h, followed by treatment with various concentrations of TB m A-Glu (0, 0.5, 1.0, 2.0, 2.5, and 5.0 M) for 12 h

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The senescence problem To appreciate the significance of FOXO4-DRI, it helps to understand cellular senescence and why it matters in ageing and disease