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After three washes with phosphate buffer saline Tween (PBST) for 15 min, the PVDF membrane was incubated with secondary antibody (1:10000) at room temperature for 1 h

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Cell viability assays The cells were plated in triplicate in 96-well plates (4000 cells cultured in 2%FBS DMEM and 2000 cells cultured in 10% FBS DMEM)