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Briefly, Lipofectamine 3000 reagent was diluted in 125 l of Opti-MEM medium (#31985062, Thermo Fisher, USA)

Chemotherapy resistance to taxol in clonogenic progenitor cells following transduction of CD34 selected marrow and peripheral blood cells with a retrovirus that contains the MDR-1 chemotherapy resistance gene

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However, both Siglec-1 + marginal zone and MARCO + metallophilic macrophages (MZM and MMM) 58 formed similar structures in the spleen of Gclc fl/fl Mb1-Cre + mice compared to controls (Supplementary Fig