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The vehicle, excipients, injection volume, and delivery device can all influence local and systemic tolerability

Br J Dermatol 1997

For ankle injuries specifically, subcutaneous injection near the affected area at 0.25 to 0.50 mg daily for 4 to 8 weeks represents the most common protocol among researchers and biohackers

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However, significant data gaps remain regarding systemic absorption, multi-organ metabolism, long-term exposure, and potential liver-related risks