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The diluent is added slowly against the inside wall of the vial rather than directly onto the powder, and the vial is swirled gently rather than shaken vigorously, because mechanical agitation can stress peptide structure and generate foaming

Purity is achieved through an advanced distillation process including fermentation, centrifugation, cell extraction, filtration and crystallization

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Bound C1q or deposited C3, C4 or C5 was detected using specific primary antibodies from rabbit (all from Dako/Agilent, A0135, A0062, A0065, A0056) (diluted 1:5000 in PBS/T/S) and a secondary HRP-conjugated anti-rabbit IgG antibody from donkey (Cytiva Life Sciences, NA935) (diluted 1:10.000 in PBS/T/S)