& Pompliano, D
10.1016/j.ijdevneu.2016.08.005 Int
Heres the formula we use: Total Peptide in Vial: 5mg (which is equal to 5000mcg) Volume of BAC Water Added: This is your variable (e.g., 1mL, 2mL, 2.5mL) Desired Dose: The amount of peptide needed per application for your research protocol
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Ed Campbell, Loyola University Chicago) and Vero-E6 (ATCC CRL-1586) cells were maintained in DMEM-10% FBS (Dulbeccos Modified Eagle Media, DMEM) containing 10 mM HEPES, 100 nM sodium pyruvate, 0.1 mM non-essential amino acids, 100 U/ml penicillin G, and 100 g/ml streptomycin, and supplemented with 10% fetal bovine serum (FBS, Atlanta Biologicals, USA)
Protocol adjustments should follow systematic observation rather than impulsive changes