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Palmitoylation of cysteine residues located at the N-terminus of GAD65 is required for the transport of GAD65 to synaptic terminals [51], and decreased palmitoylation impairs GABAergic neurotransmission, as observed in Huntingtons disease [52]

The lysis buffer was prepared by mixing 10 ml of M-PER (ThermoFisher) with 100 l of Protease & Phosphatase inhibitor mix (ThermoScientific) and 100 l of 0.5 M EDTA (ThermoScientific)

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Even if its safe in small doses, we have no data about what happens at higher doses