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A catalogue of biochemically diverse CRISPRCas9 orthologs

This work was supported and funded by Kuwait University Research Project No

Assay of enzyme activity The rhGPx3 mutant showed activity of 25.0 U/mg and 8.5 U/mg using H 2 O 2 and 1-palmitoyl-2-(13-hydroperoxy-cis-9, trans-11-octadecadienoyl)- L-3-phosphatidylcholine (PLPC-OOH) as an oxidizing substrate, respectively (Figure 2)