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While at the 28th day, a diversity of muscle fiber types still persists resulting in different PAS staining (PAS intensity staining + and +++), at 42nd day this diversity vanished, only residual muscle fibers with low PAS intensity staining were observed (Figure 14a,b)

Lin et al., 2020)

However, the IM route was the top-ranked statistically, followed by the SL and then the oral routes, but without clinical significance

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Answer: There is no strict upper limit, but most protocols build in breaks after 12 to 16 weeks of continuous use to limit tolerance

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