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both antibiotic use and germ-free conditions suppress the expression of CYP7A1 and CYP27A1, key enzymes involved in BA production based on animal mechanistic studies (Chiang and Ferrell, 2019)

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The mobile phase was 5 mM sulfuric acid in water (Milli-Q grade) with an isocratic flow rate of 0.8 mL min 1