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The application of melanotan 2 in this context may have implications for the treatment of obesity, especially in MC4R-deficient subjects who are prone to the disease [12, 13, 14]

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Individuals with obesity are at elevated risks of diabetes, cerebrovascular disease, and solid cancer

The resulting supernatant was analyzed using high-performance liquid chromatography (HPLC, VWR Hitachi, Radnor, Pennsylvania, USA) coupled with mass spectrometry (3200 Q Trap, AB Sciex, Framingham, Massachusetts, USA) to quantify the target compounds