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This represents direct evidence of the mechanism at the cellular level, though its translation to clinical longevity outcomes in humans continues to be studied
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Because it lacks the drug affinity complex, it does not covalently bind serum albumin and its action remains short, which is precisely what makes it useful for studying pulse shape rather than sustained elevation
One reason for this is that the level of glutathione will fluctuate throughout the day from its lowest in the early morning to depletion from routine exposure to toxic substances (like alcohol), pathogens, cigarette smoke, car exhaust, drugs, certain foods, stress level, illness, and many similar factors, so repeated testing could be required to set a baseline