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also, a dual effect with the anti-inflammatory IL-1ra response was observed by the Laboratory 1 only

Cell viability assay For cell viability assay, cells were planted in 96-well plates (34 10 3 per well) after indicated treatment, then incubated with 10% CCK-8 reagent at 37 C for 2 h

The scoring standard for IHC staining adopted the immunoreactive score (IRS) system [15, 16], with the specific rules as follows: the staining intensity of GGCT protein was classified into four grades, where a score of 0 indicated no staining signal, 1 indicated weak positivity, 2 indicated moderate positivity, and 3 indicated strong positivity