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2.9.3 RT-PCR using SYBR Green Primer testing was conducted using a 25-L reaction mix that contained 12.5 L of the 2x Quanti-Tect PCR SYBR Green Master Mix (Qiagen, Germany), 0.25 L of Revert-Aid Reverse Transcriptase (200 U/L) (Thermo Fisher), 0.5 L of each primer of 20 pmol, 8.25 L of water, and 3 L of RNA template

One of these studies tGSH and tGSH:GSSG were significantly lower (by 10.7% and 14.9% respectively) in children with autism who were heterozygous or homozygous for the delta aminolevulinic acid dehydratase (ALAD) 177 GC mutation, whereas there was no difference in GSSG [50]

Acknowledgements We thank Yumeng Shen (Public Platform of State Key Laboratory of Natural Medicines, China Pharmaceutical University) for her assistance with flow analysis

Aging Cell 11 , 277283 (2012)