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A maximum of four burr holes, two in each hemisphere, were used to inject pLKO_eGFP_Lox-STOP-Lox_Gsto2_shRNA or scrambled-shRNA viral vector with the following stereotaxic coordinates: anterior injection (anteriorposterior (AP): 0.95 mm

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The cost of a degraded dose far exceeds the cost of a small amount of discarded peptide