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For immunofluorescence, sections were washed with PBS at room temperature twice for 10 min, followed by incubation in blocking buffer (5% fish gelatine, 0.5% Triton in PBS) at room temperature for 1 h before incubation with the primary antibodies (see Supplementary Table 4 for primary antibodies used in this study) diluted in the blocking buffer at 4 C overnight

showed that administration of Aib2 C24, another dual GLP-1/GCG receptor agonist, alongside HFD feeding, prevented the development of hepatic steatosis, inflammation, and fibrosis in mice [44]