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For cell culture experiments mouse TM3 Leydig cells and GC-1 spg spermatogonia cells were utilized

However, there are a few things to keep in mind: Zinc Levels: Some studies suggest that long-term, high-dose glutathione supplementation may affect zinc levels

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Impacted scaffolds were returned to a 6-well plate after impact and incubated at 37 C, 5.0% CO 2 , with daily media changes, until imaging

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