Proteins are long chains (hundreds to thousands of amino acids) that fold into complex structures to perform roles such as enzymes, structural support, transport and immune function
Together, these actions promote tumor proliferation and metastasis
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Cells were then lysed in 1X TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) supplemented with 1 mM EDTA, 1% Triton X-100, phosphatase inhibitor cocktail (P5726, Sigma Aldrich), and protease inhibitor cocktail (11873580001, Roche Diagnostics)
Wu 2012)