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The ELISA plate was washed and blocked with I-BlockTM (Applied Biosystems) buffer (1 PBS + 0.2% I-Block + 0.05% Tween20) for 1 h at room temperature

10.1016/S0006-291X(75)80062-3

It is notable that there was an increased pregnancy failure in the aged female mice following successful mating regardless of melatonin treatment, as indicated by the presence of mating plugs and mice bearing pups

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