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In a 96-well microtiter plate, 20 l of cell lysate was prepared in each well

These 15 altered metabolomics pathways resulted in increased levels of glutamate, 4-Aminobutanoate, carnosine, thymine, porphobilinogen, homogentisate and 4-Guanidinobutanoate in the R group ( Figure 5B , Supplementary Figure 4A )