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Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected

Before using any peptide in a research model, consult the relevant institutional review board, animal-care committee, and biosafety office

10.1096/fj.202202108R 168 WuY.TangZ.DuS.DaiJ.ShenJ.NiuH.et al

Furthermore, GLP-1RAs have been shown to attenuate microglial activation and improve memory-related behaviors

-amyloid-induced migration of monocytes across human brain endothelial cells involves RAGE and PECAM-1

While research stage currently, these innovations may improve convenience and compliance within 5-10 years