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Prioritising Sleep Research suggests that chronic sleep deprivation can deplete glutathione levels

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For each treatment 15 g of total proteins extract from the leaves were loaded on SDS-PAGE (12% acrylamide) and immunodetected with anti-GPX (Agrisera)

E., Bartlett, P

Isolation of cytosolic, mitochondria and nuclear fractions Mitochondria or nuclear fractions were immediately extracted using the Mitochondrial or Nuclear Isolation Kit (Beyotime), respectively

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