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Images were analysed in real-time on a global scale using the built-in Leica Application Suite X (LAS X) for FLIM, with lifetime calculated with fitting to the in-built model of mono-exponential reconvolution

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Mediated by the TOM complex, PINK1 undergoes trans-autophosphorylation and subsequent conformational rearrangement, thereby activating the E3 ubiquitin ligase activity of Parkin through its transition from an autoinhibited state to an active conformation (Riley et al., 2013)

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doi: 10.1002/jcla.23994