Immunocytochemistry Immunolabeling of NPCs and derived neurons was accomplished by utilization of a standard two-day protocol with: 15 min fixation in 4% PFA, 10 min permeabilization in 0.1% triton-PBS and 30 min blocking with 5% donkey serum
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Rapa SF, Di Iorio BR, Campiglia P, Heidland A, Marzocco S
This review aims at providing a broad view of PAIS, ranging from the molecular/cellular level to the whole organism, to provide a theoretical background and to introduce notions that could help understanding how PAIS can have both clear core features and a large set of diverse symptoms
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No amount of glutathione can outrun a total lack of sleep, and no amount of glutamine can replace a balanced diet