Among those strategies are glutamine mimicking compounds (DON and JHU083), glutamine transporter blockers (GPNA, V-9302, and JPH203), major glutamine-producing enzymes (CB-839 and BPTES), glutamine depletion (L-asparaginase), and multiple downstream actors of glutamine biosynthesis [12,14,52]
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Its most extensively studied molecular property is G-actin sequestration : Tb4 binds monomeric actin and participates in the regulation of the actin cytoskeleton, the protein scaffold that drives cell shape and, critically, cell migration
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The solution was then centrifuged at 200 x G for 2 min at 4 C to pellet host cell debris
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