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Five mL of the pre-culture were added to 450 ml LB medium and grown at room temperature to an OD 600 of 0.50.8

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Dopaminergic pathways are similarly affected, with reduced tyrosine hydroxylase activity leading to diminished dopamine in the basal ganglia and prefrontal cortex, manifesting as anhedonia, reduced motivation, and psychomotor retardation (73, 74)

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