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Spectrophotometric characterisation of the Cu(II) centre, stability studies across buffer compositions, competition experiments against other chelators, and method development for HPLC or ICP analysis all consume modest amounts of material while depending heavily on the complex not having degraded in storage

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Here it is also useful to note that vegetarianism, and any concomitant vitamin B 12 deficiency, have presumably been around for several centuries in the Indian subcontinent, while the growth of diabetes is relatively recent in the last few decades