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At this concentration every 0.25 mg of dose is 0.1 mL, which is 10 units on a U-100 syringe

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Taking readings at the same time each day, ideally in the morning before any activity, creates a baseline against which changes can be assessed

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Surface marker staining was performed using CD45-BV786 (clone 30-F11), CD41-PE (MWReg30), CD11b-AF700 (M1/70), and Ly6G-PE-Cy7 (1A8) for 30 min at 4 C