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(2), with pGCG = plasma glucagon level, k = slope of the regression line at baseline of SUV 55min (SUV 55min, Baseline ) over plasma glucagon (pGCG Baseline ) Estimation of slope k was based on all n = 13 subjects for whom SUV 55min and plasma glucagon values were available at baseline

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The reasoning is simple: too little is easy to add to

However, the aforesaid method suffers from disadvantages such as a difficulty to perform recrystallization and high production costs due to the use of an expensive optical resolution reagent

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