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Measurement of reactive oxygen species (ROS) and reduced glutathione (GSH) After maturation, the oocytes were incubated in 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) and 10 M Cell Tracker Blue (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 37 C in the dark to detect intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), respectively
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Results Exposure of MCF-7:2A cells to 1 nM E 2 plus 100 M BSO combination for 48 to 96 h produced a sevenfold increase in apoptosis whereas the individual treatments had no significant effect on growth
Withdrawal is a neurological event driven by the brains chemical adaptation to alcohol
