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J Biol Chem 245:26122621 Smith TJ, Bell J (1985) Investigation of the effects of crosslinking glutamate dehydrogenase with dimethylpimelimidate

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Dynamic data was first fit into kinetic models with T 1 as an unknown parameter to estimate apparent in vivo T 1 of VitC, which was subsequently incorporated into the kinetic model as a fixed parameter to generate k assuming T 1 is the same across the subjects