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The underlined sequences in the primers correspond to Kpn I and Xho I restriction sites for subcloning

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NIX can directly bind to LC3 in the BH3 domain, recruiting phagosomes (precursors of autophagosomes) to mitochondria and acting as autophagy receptors, mediating mitochondrial clearance after mitochondrial damage and red blood cell differentiation [71]

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Ando, T., Latif, R