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RNA quantity and quality (260/280 and 260/230 ratio) was measured by a MaestroNano Spectrophotometer (Maestrogen, Taiwan) and RNA degradation was also checked by formaldehyde agarose gel electrophoresis of total RNA

The median total dose (including all administrations from pre-surgical PK and loading doses to post-hospital follow up) was 629 IU/kg (range: 464 1,457 IU/kg) for major orthopedic surgeries, 489 IU/kg (range: 296 738 IU/kg) for major non-orthopedic surgeries

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Cross-reactive epitopes may trigger parallel autoimmune responses in multiple organ systems, explaining why HT often appears as part of a broader systemic autoimmune network (Betterle et al

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